WG1: Research Needs

WG1 deals with the requirements (basic, physiological, behavioural, etc.) of cephalopods species (and the research behind) to fit in compliance with the aims and principles stated in Directive 2010/63/EU. Many members of the MC will be part of this WG. The implementation of TS will be particularly important in WG1, to let users at different levels practice and experience the procedures compliant with Guidelines and Directive, and test and compare devices, methods and protocols that will help the application of the 3R principle to  cephalopods. STSMs are also central in WG1, they will strengthen the collaborations among partners and allow the development of task-related research projects, in particular to evaluate the possible extension and relative requirements of other cephalopod species to the Guidelines available, thus to facilitate the spread of the portfolio of species to be utilized in research and training and the possible actions for the establishment of alternatives. The biological diversity of cephalopods (including the level of behavioural repertoire), reflected in different physiological adaptations to varying conditions is not consistent with a “universal” welfare guidelines concept. The establishment of acceptable welfare guidelines specific for cephalopod species is a prerequisite for the implementation of the 3R principle in cephalopod research. The publication of the achievements of Short Term Scientific Missions and Training Schools in peer-reviewed publications will be an important component of WG1 dissemination.

This Work Group will integrate the following tasks:

Task 1 – Guidelines and experimental practices improvement

Task 2 – Species-specific research procedures 

Task 3 – New technologies and experimental approaches to promote the 3R’ principle.

The main objectives of this Working Group are: To determine best laboratory practice and implement the 3R principle, as it is essential to fill the gaps of knowledge on cephalopod welfare. Hence this group will address the following research needs:
1. Environmental need for different cephalopod species: tank size and space requirements, water quality, light conditions; trial with different parameters to reduce stress and increase welfare.
2. Nutritional requirements of different cephalopods species at various developmental stages: proteomic study of cephalopods paralarvae, trial of pre and probiotics to improve health.